plasmids for flavidin Search Results


plasmids for flavidin  (Addgene inc)
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Addgene inc plasmids for flavidin
Validation of <t>Flavidin</t> Thermostability and Ligand Binding (A) Flavidin thermostability after heating for 3 min in PBS at the indicated temperature and then analysis of tetramer integrity by SDS-PAGE with Coomassie blue staining. C is a control boiled in SDS before loading. (B) Biotin-4-fluorescein association rates <t>for</t> <t>Flavidin</t> with or without 635P-NHS labeling (mean ± 1 SD, n = 9). (C) Biotin-4-fluorescein dissociation rates for Flavidin with or without 635P-NHS labeling (mean of triplicate ± 1 SD). See also <xref ref-type=Figure S4 . " width="250" height="auto" />
Plasmids For Flavidin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Validation of Flavidin Thermostability and Ligand Binding (A) Flavidin thermostability after heating for 3 min in PBS at the indicated temperature and then analysis of tetramer integrity by SDS-PAGE with Coomassie blue staining. C is a control boiled in SDS before loading. (B) Biotin-4-fluorescein association rates for Flavidin with or without 635P-NHS labeling (mean ± 1 SD, n = 9). (C) Biotin-4-fluorescein dissociation rates for Flavidin with or without 635P-NHS labeling (mean of triplicate ± 1 SD). See also <xref ref-type=Figure S4 . " width="100%" height="100%">

Journal: Cell Chemical Biology

Article Title: Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction

doi: 10.1016/j.chembiol.2017.06.015

Figure Lengend Snippet: Validation of Flavidin Thermostability and Ligand Binding (A) Flavidin thermostability after heating for 3 min in PBS at the indicated temperature and then analysis of tetramer integrity by SDS-PAGE with Coomassie blue staining. C is a control boiled in SDS before loading. (B) Biotin-4-fluorescein association rates for Flavidin with or without 635P-NHS labeling (mean ± 1 SD, n = 9). (C) Biotin-4-fluorescein dissociation rates for Flavidin with or without 635P-NHS labeling (mean of triplicate ± 1 SD). See also Figure S4 .

Article Snippet: Requests for plasmids for Flavidin (pET21-Flavidin, https://www.addgene.org/89881/ ) and K121R mutant (pET21-Streptavidin-K121R, https://www.addgene.org/89880/ ) may also be made from Addgene.

Techniques: Biomarker Discovery, Ligand Binding Assay, SDS Page, Staining, Control, Labeling

Journal: Cell Chemical Biology

Article Title: Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction

doi: 10.1016/j.chembiol.2017.06.015

Figure Lengend Snippet:

Article Snippet: Requests for plasmids for Flavidin (pET21-Flavidin, https://www.addgene.org/89881/ ) and K121R mutant (pET21-Streptavidin-K121R, https://www.addgene.org/89880/ ) may also be made from Addgene.

Techniques: Virus, Recombinant, Avidin-Biotin Assay, Blocking Assay, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Sequencing, Software